Determination of Reference Range of Glycosylated Haemoglobin (HbA1c) for Different Age Groups
Objective: To determine the reference interval of glycosylated haemoglobin for different age groups in our local Pakistani population.
Subjects & methods: Total number of 164 healthy adults (91 females and 73 males), were selected through nonprobability consecutive sampling. After informed consent, venous blood sample (3-4 ml) from each subject was collected in EDTA vial under aseptic conditions. Haemolysed blood was used for estimation of HbA1c by Fast Ion-Exchange Resin Separation method on Microlab 300. The participants of the study were divided into three groups according to their age distribution. All data was analysed using Microsoft Excel and statistical software Minitab15. Mean and SDs were calculated for each group. Reference range of HbA1c was calculated for each group by using the formula Mean ± 2SD.
Results: One hundred and sixty-four healthy individuals were included in the study. Mean age of the individuals was 32±11.6 years and mean HbA1c level was 4.17±0.93%. The reference range of HbA1c for age group 20-30 years was 2.09-5.57% with the mean value of 3.83±0.87%. The reference range of HbA1c for age group 31-40 years was 2.63-5.99% with the mean value of 4.31±0.84%.The reference range of HbA1c for individuals > 40 years of age was 2.8-6.4% with the mean value of 4.31±0.9%.
Conclusion: For proper assessment of the diabetic status, it is mandatory that every lab should establish its own reference range of HbA1c for both genders and different age groups representing its patient population.
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