Role of Nigella sativa in Carbon Tetrachloride induced Hepatotoxicity

  • Kashif Shaikh Assistant Prof. Pharmacology, Muhammad Medical College, Mirpurkhas
  • Majid Ali Hingoro Assistant Prof. Pharmacology, Islamabad Medical & Dental College, Islamabad.
  • Amin Fahim Associate Prof. Histopathology, Al-Tibri Medical College, Karachi
Keywords: Carbon tetrachloride, Hepatoprotective, Hepatotoxicity


Objective: To evaluate the protective role of Nigella sativa in carbon tetrachloride induced hepatic changes in rabbits.
Material and Methods: This Case control experimental study was conducted at Department of Pharmacology, ISRA University Hyderabad during July 2011 to November 2011. A total of 45 rabbits were divided into three groups consisting of 15 animals in each group A, B and C. Each group was further divided into three sub groups. Sub groups (A1,B1 and C1) received treatment for one week. Sub groups (A2, B2 and C2) received treatment for two weeks. Sub groups (A3, B3 and C3) received treatment for three weeks. The animals in group A (control) received normal saline. The animals in group B were treated with carbon tetrachloride. The animals in group C were treated with carbon tetrachloride and Nigella sativa.
Result: In present study sinusoidal congestion, periportal inflammation, kupffer cell hyperplasia, steatosis, necrosis and fibrosis were seen in carbon tetrachloride intoxicated rabbits. These findings were less marked in rabbits treated with Nigella sativa.
Conclusion: This study showed the hepatoprotective effects of Nigella sativa in carbon tetrachloride induced hepatotoxicity. 

Original Articles