In-Vitro Analysis of Murraya Koenigii Root extract with Colistin Against MDR Pseudomonas Aeruginosa

Abstract

Objectives: To analyze susceptibility of MDR Pseudomonas aeruginosa to chloroform root extract of
Murraya koenigii.
Methodology: For this research Murraya koenigii chloroform root extract (MKCRE) was obtained from PCSIR, Lahore
with total yield of 3.4%. A 14-day study was conducted on blood samples of 12 patients admitted to Sheikh Zayed
Hospital (SZH) Lahore over a period of one year, assessed positive for MDR P. aeruginosa based on a series of
confirmatory bench tests performed in the Microbiology lab of SZH Lahore. Simple Randomized sampling was done
by rotary method, and the samples were randomly divided into 2 groups, each group having 6 samples. Groups 1 & 2
were tested for susceptibility to MKCRE and Colistin respectively using Disk diffusion and Agar well diffusion methods
for determination of zone of inhibition and MIC (Minimum Inhibitory Concentration) was determined by broth
macro-dilution method. Data was statistically analyzed using SPSS version 25.
Results: Our study concluded that MKCRE have significant anti MDR P. aeruginosa activity with pharmaceutical
potential.
Conclusion: The current study revealed that Murraya koenigii chloroform root extract individually carries Anti MDR
Pseudomonal potential is better than Colistin.
Keywords: Murraya koenigii root extract, Colistin, Pseudomonas aeruginosa