Combined Effect of Alpha Tocopherol and Ascorbic acid on Body Weight of Rats Exposed to Chronic Restraint Stress

  • Sadia Moazzam Professor of Physiology, Fazaia Medical College.
  • Meena gul Associate Professor, Department of Physiology, GKMC, Swabi.
  • Shemaila Saleem Associate Professor, Department of Physiology, Federal Medical& Dental College
Keywords: Chronic stress, Ascorbic acid, Body weight, Alpha tocopherol,


Objective: This study was planned to determine the combined effect of ascorbic acid and alpha tocopherol on body weight of Sprague Dawley rats exposed to chronic restraint stress. Materials and Methods: The study was conducted in National Institute of Health (NIH), Islamabad. Ninety male Sprague Dawley rats (mean wt 250 ± 50 grams) were used and divided into 3 groups. Each group comprised of 30 rats. Group I was taken as control. Group II was exposed to chronic stress, whereas Group III was given combined doses of ascorbic acid and alpha tocopherol prior to restraint stress. Results: The comparison of weight gain by the different groups revealed that there was a significant decrease in weight gain of rats exposed to the chronic stress as compared to control group. Combined supplementation with ascorbic acid and alpha tocopherol resulted in significant improvement of their eating habits. Conclusion: Chronic stress has a detrimental effect on weight. This effect can be minimized by the intake of sufficient amount of antioxidants. 

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