Distribution of Extended-spectrum β-lactamase and Metallo-β-lactamase-producing Pseudomonas aeruginosa in Tertiary Care Hospitals of Lahore, Pakistan

Syeda Aneela; Noor- ul-Ain; Samyyia Abrar; Muhammad Saeed; Shahida Hussain; Abdul Hannan; Saba Riaz

doi:10.35787/jimdc.v8i1.312

Syeda Aneela Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore
Noor- ul-Ain Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore
Samyyia Abrar Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore
Muhammad Saeed Medical Lab Technologist-Manager Pathology, DHQ Hospital Mandi Bahauddin, Primary & Secondary Health Care Department, Government of Punjab, Pakistan.
Shahida Hussain Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore
Abdul Hannan Professor, Department of Microbiology, University of Health Sciences, Lahore
Saba Riaz Assistant Professor, Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore

DOI: https://doi.org/10.35787/jimdc.v8i1.312

Keywords: ESBL, MBL, MDR, Pseudomonas aeruginosa, DDST

Abstract

Background: Pseudomonas aeruginosa (P. aeruginosa) is an important bacterial pathogen most frequently associated with nosocomial infections, especially in immuno-compromised patients. Early detection of these life threatening, β-lactamase producing bacteria is essential for infection control and to prevent their dissemination. The aim of our study was to detect the presence of Extended-Spectrum β-Lactamase (ESBL) and Metallo-β-Lactamase (MBL) strains of Pseudomonas aeruginosa.
Material and Methods: Eighty-eight identified strains of P. aeruginosa were collected from Chughtai Laboratories, Combined Military Hospital and Children Hospital, Lahore. These strains were sub-cultured and after confirming the cultural characteristics by Gram staining and colony morphology, manual biochemical identification was done. Susceptibility to various antibiotics and production of extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs) were determined using modified Kirby Bauer disk diffusion method, double disk synergy test, combined disk synergy test (CDST) and inhibitor-potentiated disk diffusion test (IPD) respectively.
Results: Out of eighty-eight strains tested, three were ESBL producers (3.4%) and eleven strains (12.5%) were found to be resistant to carbapenems. Of these, eight were MBL producers (72.7%). All these β-lactamase producing strains (14 strains) were multidrug-resistant (MDR). Piperacillin and piperacillin/tazobactam proved to be the most effective antibiotics in both types of β-lactamase producing strains.
Conclusion: Our study shows noticeable emergence of β-lactamases (ESBLs & MBLs) in P. aeruginosa. All of these strains were MDR. It reveals a correlation of these β-lactamases with multidrug resistant genes.