In-Vitro Toxicology Approach to Explore Nicotine Effects in Oral Mucosal Culture

  • Muhammad Nauman Sheikh Associate Professor, Department of Oral Pathology, College of Dentistry
Keywords: Nicotine, Oral mucosa, Tobacco, Viability of cells

Abstract

Objective: To measure the effect of in vitro nicotine on reconstituted oral mucosal culture and to study its effect after 5 minutes and 24 hours in normal healthy uninflammed oral mucosa.
Material and Methods: This observational study was conducted at Department of Oral Pathology, Barts and London Queen Mary University London to measure the effect of in vitro nicotine on reconstituted oral mucosal model. The reconstituted human epithelium model was used and was supplied by Skin Ethic Laboratories, Nice, France. Cells viability was assessed by MTT assay. Working solutions (10μM, 100µM, 1mM, and 10mM) of nicotine were primed from 2.5M stock solution (Sigma,UK). The effect of nicotine was studied after 5 minutes and 24 hours in normal healthy uninflamed oral mucosa.
Results: It was found that that application of nicotine after 5 minutes and 24 hours’ treatment on uninflamed oral mucosal model and did not significantly affect the viability at all concentrations used.
Conclusion: Nicotine did not show any effect on uninflamed mucosa and also had no momentous effect after 5 minutes and 24 hours respectively. Further workup on proteomics and genomics is suggested to confirm our observations.

Published
2017-09-15
Section
Original Articles