Histo-Pathological Effects of Raphanus Sativus Leaf Extract on Carbon Tetrachloride Induced Hepatotoxicity

  • Ahsan Aslam Senior Lecturer, Department of Pharmacology, Isra University Hyderabad
  • Muhammad Saleh Chief Medical Officer, Casualty outdoor patient department, Liaquat university Hospital
  • Farzana Rahim Memon Assistant Professor, Department of Physiology, Isra University Hyderabad
  • Sadia Khan Lecturer, Department of Biochemistry, Isra University Hyderabad
  • Soobia Pathan Medical officer, PPHI
  • Kazbano Ramsha Lecturer, Department of Biochemistry, Isra University Hyderabad
Keywords: Carbon Tetrachloride, Raphanus, Oxidative Stress


Background: Raphanus sativus is a cruciferous plant which possesses free radical scavenging properties that defend the body against the deleterious effects of reactive oxygen species. The study objective was to assess the protective effect of Raphanus sativus in carbon tetrachloride induced hepatotoxicity in albino Wistar rats.

Methodology: This animal based experimental research was conducted from September 2019 to March 2020 at the Postgraduate Research Laboratory, Isra University, Hyderabad. Non-random purposive sampling technique was used for selection of study animals. Rats were distributed evenly in three different groups: Group 1 (control group), Group 2 (CCl4 induced experimental group), and Group 3 (CCl4 plus Raphanus sativus treatment group). Data was analyzed using SPSS version 24.

Results: Liver weight and serum markers of hepatic function were high in group 2 as compared to group 1 and C rats (p<0.05). Treatment with Raphanus Sativus significantly reduced serum levels of LFTs (p<0.05). There was a significant decline in the plasma levels of oxidative markers in group 2 while marked histo-pathological changes like necrosis, sinusoidal dilatation and congestion observed among animals of group 2.

Conclusion: Raphanus Sativus wields anti-oxidative as well as hepato-protective effect against carbon tetrachloride induced hepatotoxicity and tissue damage.

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